I am analysing two compounds which show an overlap between the higher wavelength end of the excitation spectrum and the lower wavelength end of the emission spectrum (one compound with excitation wavelength at 280 nm and emission wavelength at 300 nm) , the other one with excitation wavelength at 290 nm and emission wavelength at 304 nm
i have two questions:
1- can these emission wavelengths be used for compounds quantification in presence of this overlap (risk of interference between excitation and emission wavelengths at the detector)??
2- if not , what is the instrumental manipulation that can be done to solve this problem to achieve better separation between the two spectra( excitation and emission)??