At first I thought it was the column guard, but when directly connect to the column it still comes off. Then I thought it was the column, so I switched columns and the same thing keeps happening. Need help.
Does it come off when you put Flow on it ?
If yes then you could try to put low flow for a while and increase from time t time.
You also can check if you are using the right connectors to it.
Did the problem occur on a already established column.
Then the other question is. what did you change recently ? Check every new part. Sometimes I get confused with the Part.Nr.
Thanks for the response!
Yeah when I put flow through it the line comes off, but only after a few seconds, the solvent is going through the column.
This is already an established set up and nothing different has changed, the only difference is that I changed my buffer system.
However, before the problem started occurring, after one of my runs was complete the pump was not pushing any liquid through. So I removed the pump head and cleaned it out, and washed the system with methanol and water, thinking that there was a clog somewhere.
When the column guard is not attached to the column the line doesn't come off, only when the column is also connected.
So starting at a low flow rate and increasing it periodically appears to have fixed the issue, I did this with methanol.
There might have been a clog somewhere else in the instrument.
Thanks so much!
Are you using peek fittings? I normally use them on the out of the column, because they come off when the system reaches about 200 bar. For the inlet of the column I recommend the A-line fittings or a stainless steal finger tight. If you iuse the A-line fittings remeber you will need the correct capillary.
A-Line Quick Connect Fitting - Agilent’s A-Line Quick Connect Fitting Wins TASIA Award | Agilent
The fittings have been the same ones we have been using for a while now.
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