We have 6490A LCMS. Instrument works well but when I checked for autotune it failed. The data seems OK but now days I have to autotune every two weeks. Can anybody advise if I am over killing (by too many times autotuning) or it is a signal of instrument breakdown down the road!
How often do you run checktune? You should checktune to look at mass assignment, peakwidth and has abundance counts. You should only autotune when you are not getting good checktune results.