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can modification of amino acid(or base) influence the result (migrate time and/or molarity) of Agilent 2100?

Question asked by jingbozhao on Dec 18, 2018
Latest reply on Dec 19, 2018 by jingbozhao

1. Can modification of amino acid (or base) influence the result (migrate time and/or molarity) of Agilent 2100?

2. Using DNA 1000 kit, I found Agilent 2100 can detect the ssDNA, but with a wrong size (or concentration, not sure yet). About the wrong size peak, could it be any different if I increase the ssDNA input or use a modified ssDNA (5'-phosphorylation e.g.)?

3.What about the Y-adaptor used in NGS? the migrate time is faster or slower than dsDNA?

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