4 Replies Latest reply on Dec 12, 2018 2:03 PM by staceylouie

    G1321B FLD Leaks

    staceylouie

      Hello, in our lab we have gone through two FLD flow cells that developed leaks in the past month. The first had a crack in the quartz cell, and the second cell appears to be leaking right behind the inlet port but does not have any leaks in the quartz cell. The FLD is being used on a 1290 system (G4220A, G4226A, G1316C, and G4212B upstream, no detector downstream).

       

      Aside from user error, I was wondering if any of the scenarios we are running could be more risky in terms of developing a leak?

      • Is the cell particularly fragile depending on the type of solvent (water, PBS, acetonitrile, methanol, ethanol), or solvent exchanges between these?
      • Pump purging / Pump conditioning -- Does the FLD need to be disconnected when either of these is done?
      • Would the inlet port leak be caused by an over-pressure (e.g. excessively high flowrate), or would this be some other problem?

       

      If there are any other tips that we can incorporate into the user training, it would certainly be appreciated.

        • Re: G1321B FLD Leaks
          howwhite

          Hi stacylouie,

           

               Sorry to hear about the leaking flow cells !  The cell is fragile - maximum permissible back pressure is just 20 bar. A couple of things must  be done to avoid rupturing the flow cells - first and foremost be sure to use ONLY the correct waste tubing supplied in the accessories kit G1321-68755. Do Not substitute any waste line from another detector as these may cause excessive backpressure to the cell resulting in ruptures or leaks ! 

              During Purging, Priming and Conditioning of the pump flow should be directed to waste only, not downstream through the sample path.

           

          Hope this helps ! 

          1 person found this helpful
            • Re: G1321B FLD Leaks
              staceylouie

              Thanks, I will pass this information along to the students.

              We have also received a lot of different information regarding whether conditioning can be done with vs. without the column on -- would you be able to advise on that as well?

                • Re: G1321B FLD Leaks
                  kbusch

                  It is recommended to condition with the column installed; however, it is not necessary (a union or better yet a restriction capillary can be used). Conditioning is recommended to be run at at least 200 bar and performs best above 500 bar. Be sure to keep an eye on your column limitations though. Also, conditioning should be done with a balanced composition of A and B (for example, 50%A and 50%B) that way both pumpheads and the system can be conditioned.

                  1 person found this helpful