1 Reply Latest reply on Nov 10, 2018 10:38 AM by hafizrafique

    hplc method validation_linearity

    anto

      Goodmorning to everybody,

      I'm trying to validate an HPLC method for the analysis of curcuminoids in a dietary supplement preparation.

      I read lot of papers (and ICH guidelines) about validation but I can't understand how to ensure linearity, some of you could help me?

      The excpected amount of curcuminoids is 250mg in 868 mg of sample.

       

      Thank you!!

      anto

        • Re: hplc method validation_linearity
          hafizrafique

          Linearity can be achieved through running of different known concentration of reference material. It can't be ensured by running sample due to recovery problem. I think you can made different concentrations from reference material and run on sample and then simply draw regression line. If correlation coefficient is more than 99% then linearity  of your instrument and the worker is ok.