For Normal Phase
First unplug the column an insert a blank Column.
Then flush out the Salts from the buffer. Use the same Solvent what u use for your buffer.
For Example Hexan. Use the same amount of it. then increase the Hexan. Till you reached 95 % Solvent and 5 % Water.
Then use Water / IPA. Put the pressure to 200 bar then u can purge the system with 10 ml / min.
Frequency is after every run. Best way is to write a cleaning method.Configure it that it don't give you any reports.
You just put it after every finished run.
We used 10 % ACN. The second method had 95% ACN. Let it run for 30 minutes.
After it you use 95% ACN or any other Solvent you use before.
If you have a 4 Pump System then you can put the 2 Solvents on position 3 and 4.
After the second run ( The column stay in the system ) your column is salt free and in storage conditions.
Hope this helps
Forgot to say. Open the Purge Vent when use the 200 bar / 10 ml trick.