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Question asked by dori on Sep 4, 2018
Latest reply on Sep 12, 2018 by jhagel

Hi everybody.

because of we have no further idea I now try it here.

Since a few days I have a ghost peak in my method. It's the same with 2 different LC Systems and 3 different columns.

Further I opened new bottles for each eluent compound and buffer salts (even used no salt).

The longer I flush with starting condition (15%B, organic) the higher the Peak gets so I suggest that ist a Problem with eluent A (92% buffer).

We use this Methode without any problems for years.

What else can be the cause?

Thanx indeed for any idea