1 Reply Latest reply on Sep 12, 2018 5:54 AM by jhagel

    "Ghost-Peaks"

    dori

      Hi everybody.

      because of we have no further idea I now try it here.

      Since a few days I have a ghost peak in my method. It's the same with 2 different LC Systems and 3 different columns.

      Further I opened new bottles for each eluent compound and buffer salts (even used no salt).

      The longer I flush with starting condition (15%B, organic) the higher the Peak gets so I suggest that ist a Problem with eluent A (92% buffer).

      We use this Methode without any problems for years.

      What else can be the cause?

      Thanx indeed for any idea

        • Re: "Ghost-Peaks"
          jhagel

          What happens if you do an injection with no vial number?

          When is the last time you did a PM?

          If you take the line from the pump to the column do you still see this ghost peak?  If not I would replace the rotor seal, needle and seat.

          What do you use for solvents?  And for flushing your system?