Hello every one, may peace and mercy be on all of you
I have a technical issue to discuss with. I have run a batch of pesticide samples (lufenuron) on HPLC by following the recommended and approved method. In this batch peak of all samples before and after the concerned sample comes to zero base line, but the peak of the concerned sample starts above the zero baseline and also ends above the zero baseline. The Agilent Chemstation auto integrate the peak above zero base line. The question to ask is whether should I integrate manually by drawing zero base line or follow auto integration?
This is up to you.
What detector are you using?
UV variable wavelength