My lab recently switched from CDS1 to CDS2 and one of our LC methods is now not working consistently/correctly. We need a sample prep in which 10ul from one vial is injected to the sample vial, followed by 55ul from another vial followed by mixing and a wait period before injecting. We have tried multiple things including mixing in the seat vs, the sample, vs the air as well as drawing the liquid before mixing and not drawing before mixing and we cannot seem to get consistent results through any of this trouble shooting. Could anyone give an example of a basic mixing method that has worked in case we are missing some obvious step that is causing inconsistent results. Below is the example of our sample prep method on an Agilent 1260 with CDS2 software. Thank you!