Hi, I have four isomers of Cypermethrin allmost separated, I call them Cypermethrin I, Cypermethrin II, etc.
And I have just one standard compound, called Cypermethrin, I made a solution of, for example, 4 ng/ml.
How do I treat those isomers properly in Masshunter Quant Analysis B07.01 Build 7.1.524.0?
Should I mark each Cypermethrin I, Cypermethrin II and so on to the IV, a 1 ng/ml concentration, as 1/4 of 4 ng/ml in calibration table? Is the assumption of equal EI/MS response correct for such isomers/quantitation? I also have an internal standard triphenylmethane of always 1 ng/ml here, if it does matter.
How do I report Cypermethrin in sum of 4 isomers automatically? Is there any group reporting functionality in Masshunter?
Please share a manual if it does exist on such cases of one compound and several isomers in chromatogram, I don't think this is a rare problem.