I have question about calculation of resolution in Qual MassHunter B.07.00. I tried calculate mannualy value of resolution, next I compared it with value calculated by MassHunter but results were not comparable.
Could someone help me with this? Any idea what is the problem? What is the acceptable limit for resolution?
I will be very grateful for help.
Please see below calculation.
In mannualy calculation I used two above formulas (1) & (2).
Formula 1 (used for Pharmacopoeia USP)
R = (8.055 - 7.952)/0.5*(0.09 + 0.135) = 0.92
MassHunter Qual R = 1
Formula 2 (used for Pharmacopoeia BP)
R = 1.18*[(8.055 – 7.953)/(0.039 + 0.041)] = 1.50
MassHunter Qual R = 2.2