Don't know if you have seen this app note from us. Hope this helps.
5989_5672.qxd.pdf 199.6 KB
Thanks for this. I notice you reconstitute in 50% organic solvent, this is something we do not currently do, (we reconstitute in 5% MPB, the starting conditions of our gradient, so I'm wondering if this could be part of the problem with our low sensitivity.
Thanks for the info.