AnsweredAssumed Answered

We have an Agilent 6100 single quad LC-MS. It was working fine until yesterday when I tried to use it to run an FIA analysis. Then during the run, the MSD shut down and I got an error that read: " Capillary/chamber voltage could not be maintained." Can

Question asked by awitter on Feb 20, 2018
Latest reply on Mar 19, 2019 by aravindk

Like • Show 0 Likes0
Comment • 6

We have an Agilent 6100 single quad LC-MS. It was working fine until yesterday when I tried to use it to run an FIA analysis. During the FIA run, the MSD shut down and I got an error that read: " Capillary/chamber voltage could not be maintained." Can anyone provide ideas about where to go next? I cleaned the capillary and put it back in but it didn't help. It seems that now no ionization is taking place because I can't get it to tune either. As I mentioned, this came on suddenly although it had been working fine.

No one else has this question

Outcomes

m4esti

Feb 21, 2018 10:13 AM

Hi there,

Does it work in negative ion or a lower positive voltage? Usually, the first thing I check to make sure I do not have a lot of salt build up in the source. I would carefully takeout the ion basket and clean behind the three pagoda insulators. Also remove the spray shield and clean that. It hat doesn't work its a defective capillary/chamber HV supply.

Hope this helps,

Mitch

Actions

m4esti

@ m4esti on Mar 7, 2018 4:05 AM

Hi there,

Just following up to see if any of my suggestions helped?

Mitch

Actions

awitter @ m4esti on Mar 30, 2018 2:24 PM

Hi Mitch, sorry it's taken me a while to get back with you. In short, no, it didn't help. We had a technician come and he replaced the power supply and I replaced the capillary and the cap and spring. I am still getting the same error message about the capillary/chamber voltage and I am able to tune in positive but not negative ion mode. Not sure where to go next.

Actions

m4esti

@ awitter on Apr 2, 2018 12:36 PM

What is the status of the source. Any salt build up? If not and if the capillary hasn't been taken out and the power supply replace, then the issue is more than likely the main board.

Actions

awitter @ m4esti on Apr 2, 2018 1:00 PM

No salt build up. We cleaned that and we already replaced the power supply and capillary. When the mass spec is on we don't see an increase in cap current (5-10 nA) from when it's not on. It's as though it's not getting the signal that it needs to turn on, but the chamber current is where it should be (0.8 uA).

Actions

aravindk Mar 19, 2019 12:21 AM

Hi, shutdown your LC-MS, Restart and wait for the vacuum, Press the reset button which is present in MSD, Once the vacuum is stable tune the system with negative mode first. I hope it will work. Good luck.

Actions

6 Replies

m4esti Feb 21, 2018 10:13 AM
Mark Correct
Correct Answer
Hi there,
Does it work in negative ion or a lower positive voltage? Usually, the first thing I check to make sure I do not have a lot of salt build up in the source. I would carefully takeout the ion basket and clean behind the three pagoda insulators. Also remove the spray shield and clean that. It hat doesn't work its a defective capillary/chamber HV supply.

Hope this helps,

Mitch
Like • Show 0 Likes0
Actions
- m4esti @ m4esti on Mar 7, 2018 4:05 AM
  Mark Correct
  Correct Answer
  Hi there,
  Just following up to see if any of my suggestions helped?
  
  Mitch
  Like • Show 0 Likes0
  Actions
  - awitter @ m4esti on Mar 30, 2018 2:24 PM
    Mark Correct
    Correct Answer
    Hi Mitch, sorry it's taken me a while to get back with you. In short, no, it didn't help. We had a technician come and he replaced the power supply and I replaced the capillary and the cap and spring. I am still getting the same error message about the capillary/chamber voltage and I am able to tune in positive but not negative ion mode. Not sure where to go next.
    Like • Show 0 Likes0
    Actions
    - m4esti @ awitter on Apr 2, 2018 12:36 PM
      Mark Correct
      Correct Answer
      What is the status of the source. Any salt build up? If not and if the capillary hasn't been taken out and the power supply replace, then the issue is more than likely the main board.
      Like • Show 0 Likes0
      Actions
      - awitter @ m4esti on Apr 2, 2018 1:00 PM
        Mark Correct
        Correct Answer
        No salt build up. We cleaned that and we already replaced the power supply and capillary. When the mass spec is on we don't see an increase in cap current (5-10 nA) from when it's not on. It's as though it's not getting the signal that it needs to turn on, but the chamber current is where it should be (0.8 uA).
        Like • Show 0 Likes0
        Actions
aravindk Mar 19, 2019 12:21 AM
Mark Correct
Correct Answer
Hi, shutdown your LC-MS, Restart and wait for the vacuum, Press the reset button which is present in MSD, Once the vacuum is stable tune the system with negative mode first. I hope it will work. Good luck.
Like • Show 0 Likes0
Actions

We have an Agilent 6100 single quad LC-MS. It was working fine until yesterday when I tried to use it to run an FIA analysis. Then during the run, the MSD shut down and I got an error that read: " Capillary/chamber voltage could not be maintained." Can

Outcomes

Related Content

Recommended Content