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Sample recovery issues in the SFC system

Question asked by xiaoz on Jan 9, 2018
Latest reply on Feb 27, 2018 by jhagel

Has any one experienced sample recovery issues in the SFC system? For example, if you inject 10 ug , how much can you recover at the end? I have run some samples on SFC by-passing the column, and collected all the eluent at the end, and had only <60% recovery. Tried different composition and flow rates for mobile phase solvent composition and make-up flow but not getting any better.

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