4 Replies Latest reply on Nov 13, 2017 4:34 AM by gchaplain

    HPLC Infinity 1290 II - Error code EE 6672 - No trigger signal for flash lamp problem




      We are having a problem with a HPLC we have.


      Error code EE 6672 keeps coming up on the instrument. The FLD flash lamp won't fire up to conduct diagnostic tests.


      The lamp has been replaced and instrument, software, PC has been rebooted but error is still there. Has anyone seen this error before? If so how was it resolved?





        • Re: HPLC Infinity 1290 II - Error code EE 6672 - No trigger signal for flash lamp problem



          the correct error code is 6722 (No trigger signal for flash lamp).


          This error is generated if the firmware detects less flashes than it expected in a given timeframe.

          There are multiple possible reasons for this i can think of:

          • Encoder problems
            • Dirt on encoder disks or eyes (Encoders are the black boxes which sit on top of the monochromators)
            • Bad connection between encoder and the mainboard
            • Defective encoder
          • Electronic problems
            • Could be a fault on the FLL board or the mainboard or a cabeling issue
            • Flash lamp does not fire at all


          To rule out the Encoder problems, you could switch between Pos-mode (two static wavelengthes), Ex-Mode and Em-Mode.
          The problem should only occur while taking either EM or EX Spectra. The type of spectra it is occuring on points to the defective / dirty Encoder. (The rotating one in the respective mode).


          If it is a encoder problem it is intermittent, as other error codes would be thrown first, if the encoder is completly defective!
          The encoders contain a reflecting disk with slots, if a dust grain sticks to this reflective disk on the wrong position, it will disturb the signal of the encoder.. As the encoders are not sealed, dust may enter them on rare cases.


          B.t.w. do you hear the ~74Hz buzzing sound of the flashlamp flashing while it is turned on? / While the detector is taking a spectrum?

            • Re: HPLC Infinity 1290 II - Error code EE 6672 - No trigger signal for flash lamp problem

              Unfortunately a new problem has arisen with it now that I can't do what is asked. "Emission motor friction too high" is the error now. Could the two problems be related?

                • Re: HPLC Infinity 1290 II - Error code EE 6672 - No trigger signal for flash lamp problem

                  yes definitely!


                  Probably the friction is really too high. The instrument meassures the friction during powerup by accelerating the grating mirror to a defined speed and the letting it rotate freely without any acceleration. If the grating mirror does not perform over X (i think it´s 8, but not sure) rotations during free rotation the error is generated.


                  If the grating friction is too high during operation the other error (No trigger signal for flash lamp) might be generated, as the trigger is generated once the grating passes a defined position. If this position isn´t reached in time the error is generated..


                  Too high friction could be due too different reasons, if this is a really old FLD which has been used to take a lot of emission spectras, the bearings might be worn out / the lubricant might be lost from the bearings..

                  If the FLD was dropped from some height the whole axis might be pushed through the motor as the grating mirror sitting on top of it is quite heavy


                  Standard procedure to fix this problem would be to replace the Emission-Grating/Monochromator assembly.


                  Please be aware that the firmware stores the relative position between the encoder position and the mirror position which is different for each grating, so if you replace it / dissasemble it / change the position of the encoder disc, you need to reset this information via Lab Advisor and do a wavelength calibration afterwards (also possible with Lab Advisor). The wavelength calibration requires glycogen to be present in the flow cell.. see "Wavelength calibration" here: https://www.agilent.com/cs/library/usermanuals/public/G1321-90012_FLD_USR_RevC_ENU.pdf

                  In my experience it works with water as well, but i don´t know how accurate this is afterwards.. I am not a chemist.

                  If you see an error "Index position lost" or "Index position wrong" this is total normal after replacing the grating.

                  1 person found this helpful