14 Replies Latest reply on Nov 2, 2017 6:24 AM by roy_milouot
      • Re: mass hunter
        james_jenkins

        Hi Roy,

        I updated the tags for better visibility. .

        Regards

        James

          • Re: mass hunter
            roy_milouot

            thanks

              • Re: mass hunter
                roy_milouot

                Nobody knows why?

                 

                is it possible to send the batch to someone?

                I'm enclosing a PrintScreen

                 

                no qualifiers data.png

                 

                please help..

                  • Re: mass hunter
                    tmh

                    Hi Roy,

                     

                    Most likely highlighting for outlier(s) is disabled in batch table view. You can go click the icon I highlighted in below graph and review which outliers have check boxes. When check is present outlier will highlight cells in batch table and when missing it doesn't highlight.

                     

                    • Re: mass hunter
                      joriener

                      Hi Roy,

                      are the integrated? Can you go on Manual integration and manual integrate them?

                        • Re: mass hunter
                          roy_milouot

                          naturally I updated them..

                          I guess I can manually integrate them but I don't see why

                            • Re: mass hunter
                              tmh

                              Hi Roy,

                               

                              One thing you can try is to delete intermediate files that dictate MH Quant GUI. This is done by deleting folder specified below.

                               

                              C:\Users\<NT user name>\AppData\Roaming\Agilent_Technologies,_Inc

                               

                              If this doesn't work you can send batch along with the batch to me [tomi_hamalainen (at) agilent (dot) com] via wetransfer.com or other similar service. I can then run it on my software to confirm that mine behaves the same.

                              1 person found this helpful
                                • Re: mass hunter
                                  roy_milouot

                                  Thank you Tomi.

                                   

                                  Did not find the file you talk about..

                                   

                                  I'm sending you the batch (with embedded method) directory zipped.

                                   

                                  FYI - I sent the same batch to my local distributer and he said he does not see a problem.

                                   

                                  Perhaps an update is required?

                                   

                                  I'm running B.06.00 / Build 6.0.388.0

                                   

                                  thanks again

                                   

                                  roy 

                                    • Re: mass hunter
                                      tmh

                                      Hi Roy,

                                       

                                       

                                      Thanks for the files. I double checked the batch and indeed filter settings were most likely okay. What I found out is that you're missing Outlier value for accuracy in the method.

                                       

                                      So you want to input values in specific outliers you want to use inside method editor. Here's graphs to show the effect of my adding Accuracy outlier values of 20 %.

                                       

                                      Accuracy cells not highlighted for ones that are outside commonly accepted 80-120 % range.

                                       

                                      Located Accuracy outlier in method and input value of 20.

                                      Returned to batch table, run Analyze and now outlier is highlighting Accuracy cells properly.

                                       

                                       

                                      p.s. There's no way to save manual integrations to method. Though when you perform manual integration it will be kept when you integrate/quantitate/analyze the batch,

                                        • Re: mass hunter
                                          tmh

                                          Hi Roy,

                                           

                                          My apologies for not focusing on your initial message regarding missing qualifier values. I tested this and I don't see this issue on my MH Quant B.08.00. Something that I did not have is your reference library so I used NIST14.L during my testing. Could you test it by removing reference library from the method or change it? Also my suggestion is to upgrade to the latest MH Quant version.

                                            • Re: mass hunter
                                              roy_milouot

                                              thank you.

                                               

                                              Is upgrading the version means paying for the software again?

                                              Is it not possible to downlowd an updated version?

                                               

                                              Nothing else to try about the qualifiers data? I am using this version for about three month now and never encountered this issue, only recently.. I don't know "what I pressed to make this happen.."

                                              Do you think you will be able to find out more about it if I let you take over the computer via teamviewer (if it's possible for you, of course)?

                                               

                                              Funny you mentioned the reference library - I am "stuck" on the method editor with the message error "reference library is required for deconvolution scan" 

                                               

                                              I do the following to try and resolve it - method -> setup reference library -> I choose a library from my local disc (W9N08.L) and pick a destination.

                                              The program creates the file alright but still no validation..

                                                • Re: mass hunter
                                                  tmh

                                                  Hi Roy,

                                                   

                                                   

                                                  If you still have the purple envelope that came with your MassHunter package, inside the blue box, you can activate it in agilent.subscribenet.com. That envelope contains Software Maintenance Agreement and it may still be valid if you didn't activate it when you received the software.

                                                   

                                                  You can try to re-install the software. Easiest way is to put CD in and run repair or do fresh installation.

                                                   

                                                  You can reference W9N08.L directly, without creating individual reference library from it. When I run it without the library I got that same message in method validation and it was fixed by adding reference library path that software can access in Globals Setup. If you have set it up, as I'm sure you have, then try to run software in administration mode by right-click icon on desktop and select Run as Administrator. It may simply be that when software is run natively depending on operating system it may only have user rights and depending on reference library location it can't access it.

                                                    • Re: mass hunter
                                                      roy_milouot

                                                      Hi Tomi,

                                                       

                                                      Tried repairing/reinstalling and also running as admin.. didn't work.

                                                       

                                                      I looked for what you described (purple envelope) but did not find it. on the installation disc (March 2013) there is a P/N number.. would that do?

                                                       

                                                      In any case, I noticed something - if you look at a certain compound that has a library match score (see pic 1) you also find qualifier response ratios, and if you look at a compound that is not defined with a library match score - those ratios are non-existance. if you still have my batch please try and see.. it seems to me the two are related...

                                                       

                                                      Another weird case is non accurate RT update. I encountered a problem with updating RT - the software seems to update it not on the middle like I am used to seeing.. plz see pic2

                                                       

                                                      Thank you very much

                                                       

                                                       

                                                      pic 1

                                                       

                                                      pic1 - library match score and qualifier response ratios.png

                                                       

                                                      pic2

                                                       

                                                      pic2 - RT update not set correctly.png

                                                       

                                                      Roy