When using MassHunter software for LC MS/MS does it bias/impact the experiment if different regression types (linear, quadratic, power) are used for different analytes? In other words, is the experiment negatively impacted if the calibration curve is calculated using a different equation for different compounds across samples?
Example: Using a quadratic curve to calculate sample concentrations for THC and using linear to calculate morphine concentrations. Do you need to stick to all linear equations or all quadratic equations or can you mix and match to get the best R^2 value and cals/controls in spec?
If mixing and matching is detrimental to the integrity of the experiment, why?