I have a two-component sample mixture for which my procedure implies preparing standards for both components in separate flasks. Therefore I register the spectra for both of them so that the spectra don't interfere in the reference solutions but they do in the sample solution.
Procedure says that I should calibrate the samples with the first order derivative of the spectrum for each of the relevant analytes. Where do I find the calibration and calculation options in ChemStation for this case? I use the following version of the software: UV-Visible GLP ChemStation B.05.02 (16)
Anybody any ideas where to look for this option and where to set all the factors I need for calibration and calculation?