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What should mammalian nuclear RNA look like on a Bioanalyzer RNA Nano Chip?

Question asked by kmagile on May 18, 2017
Latest reply on May 22, 2017 by mnevill

Hi,

 

I am going to be receiving some mammalian nuclear RNA and need to run this on the Bioanalyzer to assess quality.  What should I expect to see?  Should I see ribosomal peaks, but less of them than in total RNA?  Can the bioanalyzer give me an accurate RIN for nuclear RNA?  Does anyone have a picture of mammalian nuclear RNA run on an RNA Nano or Pico chip that they can share?

 

Thank you very much,

Kathie

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