I'm trying to desalt and purify by HPLC a 70 KDa protein. Is a C4 column ok? Or would a C8 be better?
The protein is soluble, not a membrane protein. My quantity of material is very limited.
I suggest a C4. It's more hydrophilic.
I hope that helps!
I went ahead and flagged the “Correct Answer” on this post. If you still need help though, please let us know and we would be happy to continue working with you.
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