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What LC column is best for a 70 KDa protein?

Question asked by testuser on Jun 6, 2016
Latest reply on Sep 19, 2017 by james_jenkins
  • Comment • 2

Hi,

 

I'm trying to desalt and purify by HPLC a 70 KDa protein. Is a C4 column ok? Or would a C8 be better?

 

The protein is soluble, not a membrane protein. My quantity of material is very limited.

 

Thanks!'

 

- Lu

testuser
testuser Jun 6, 2016 7:37 PM
Correct Answer

Hi Lu,

 

I suggest a C4. It's more hydrophilic.

 

I hope that helps!

 

- I-Shou

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