the library search score for a compound is different with different instrument

RE: the library search score for a compound is different with different instrument

smathers — Wed, 29 Apr 2020 20:12:11 GMT

As each system tunes slightly different, you will see varying scores. That being said, the larger difference you are observing most likely stems from the difference in tuning between an INERT source and the newer style HED source. If you compare the tune files between both sources, you will see how much they differ.

added to this, most library spectra was was not captured using the HED source, so your scores will definitley not line up as well, by default.

If this becomes an issue, you could create a custom library to be used for the HED source, which is a great alternative if you are not looking for unknowns.

RE: the library search score for a compound is different with different instrument

pwd — Fri, 03 Apr 2020 13:43:41 GMT

Hi,

as garyb007 said, with two different instruments (different hardware) it is impossible to get the exact match.

It is most likely related to the background created by using different hardware.

i.e. different columns or more or less used ion sources or multipliers create different height of background

RE: the library search score for a compound is different with different instrument

garyb007 — Sun, 10 Nov 2019 08:05:09 GMT

I would say this is normal, it would be impossible to exactly match spectra between 2 different instruments.