Auto calculate calibration amount in Openlab CDS

Hi Key

What does your sequence look like?  How do you create the calibration?

What I see from your screenshots doesn't look like ISTD calibration, but a calibration using C23:0 as a reference.  I may be missing something

Kindest regards

Andy

Hi Andy, sorry, it should be more of a one point external calibration, and other curves are referencing to C23:0. Not sure if this is the correct way to create, but most of our methods are created in this way. 

Technically there are four compounds to be measured, which are compound 2,3,5 and 6 in below table and the sum of these four compounds will give the result for Total PUFA. Compound 4 is the standard where we spike in for each sample.  

We would run the sample as calibration standard and clear all calibration every time. Before we run, the sample amount and ISTD amount is keyed in the table. Currently, all multipliers and dilution factors are set as 0. 

In this case, is there a way to auto-calculate the amount for Level 1 in the first picture by taking the ISTD amount we entered divided by 25.?

I can't think of a way to do exactly what you describe, but I'm sure that a custom calculation of some sort might achieve the same result.  

Kind regards

Andy

Hi Key, have you found the answer for your question? I am having the similar problem. Right now I am processing the results after completing the sequence. It would be great if I can put the standard concentration before starting the sequence. Thanks

Hey Kris, I tried doing custom calculations but I could not find the correct parameter to get the correct value. Currently I am still doing manual correction after the sequence has completed.

Hello,

If you are just trying to change the ISTD concentration you can do that for each injection of the sequence in the table. You may need to turn on the correct columns in the sequence. You can also change external standard values if needed in the sequence for your calibration injections. This can be done in the compound amounts tab if you have a processing method selected.

Marty Adams

Hello,

If you are just trying to change the ISTD concentration you can do that for each injection of the sequence in the table. You may need to turn on the correct columns in the sequence. You can also change external standard values if needed in the sequence for your calibration injections. This can be done in the compound amounts tab if you have a processing method selected.

Marty Adams