Im trying to get an internal standard calibration to work:
Usually I use an external standard calibration and that works fine. But I want to try to compensate for injection errors by adding an internal standard prior to injection.
In the peaks table I added all my analytes (just like for ext. STD). As last peak I added the internal standard. Added concentrations for calibration like Level 1, 2. ,3... 100mg/l 500mg/l 1000mg/l.... For the internal standard I added 250mg/l for each level. For the internal standard #ref ID of the other analytes I added the ID of it ISTD peak. After running a calibration sequence it shows me "internal standard curve" for the analytes in "review calibration", in contrast to the usual "external standard curve", so I guess I am on the right path.
However when analyzing samples after calibration it detects all the peaks, gives areas, but always shows "0 mg/l" for each component.
Does someone have an idea what I might be doing wrong, some extra step I am missing?
Do you know of a tutorial for EZ Chrom? I found a tutorial in this forum, but only for a different software.