We would like to record one chromatogram with two different signals:
Signal A at 220nm from 0 - 7 min
Signal B at 230nm from 7 - 27 min
The substances eluating before 7min are having their maximum of absoption at 220nm and the substances from 7 to 27 min at 230nm. We need to determine a purity using the 100% method.
When recording the Signals separately OpenLab calculates a purity for each wavelength.
It doesn´t work with the following settings:
The chromatogram always records at 220nm. Without signal change, which could be verified by peaks after 7 minutes.
The function extract maximum wavelength in data analysis is not an option.