HPLC - DAD Spektrenaufnahme

Hello Did you perform a wavelength calibration after replacing the deutarium lamp?

Hallo Haben Sie nach dem Austausch der Deutariumlampe eine Wellenlängenkalibrierung durchgeführt?

Clean flow cell by flushing with some flow at least two hour to clean the flow cell..

Reinigen Sie die Durchflusszelle, indem Sie mindestens zwei Stunden lang mit etwas Durchfluss spülen, um die Durchflusszelle zu reinigen.

In reference manual of 1100, it's mentioned how to do it.

In diagnostic option of chemstation, you can find it..

I don`t use Chemstation. I have OpenLab 2.2, but i think the work with the LabAdvisor is nearly the same. I will look, if i find the time to do something. Because i will only work the next two days and then i have holydays. So, when i am back, then i will search for the problem and then i will say, if it goes.

THANKS.

https://community.agilent.com/knowledge/lc-portal/w/lc-knowledgebase/7722/removing-and-re-installing-visible-and-uv-lamps-in-agilent-1260-infinity-g1315x-dad-or-g1365x-mwd

Above is wiki for lamp replacement and calibration by lab advisor

Thanks for the link. I will try it today.

You are most welcome...

Hello all,

I wanted to give feedback again on my question above. I was now three weeks on vacation and have thought about my problem.
Since I had been of the opinion that nothing is missing from the detector due to the nice peaks, I can now confirm this. Because via the function Isoabsorbance Plot in Data Analysis (OpenLab) the spectra are clean and nice to see. The big question now is - why are these no longer read out automatically? Because manually it is possible without problems. If anyone knows anything more about this, I appreciate any help. THANKS!

Hello all,

I wanted to give feedback again on my question above. I was now three weeks on vacation and have thought about my problem.
Since I had been of the opinion that nothing is missing from the detector due to the nice peaks, I can now confirm this. Because via the function Isoabsorbance Plot in Data Analysis (OpenLab) the spectra are clean and nice to see. The big question now is - why are these no longer read out automatically? Because manually it is possible without problems. If anyone knows anything more about this, I appreciate any help. THANKS!

Hi

Can explain what do you mean by automatically and manually.

Okay,

Automatic: Normally I go to Uv-Spectra, then I can click on the peak and automatically get the spectrum to the peak. It would also work if I mark 5 peaks together, then I get the spectra for all peaks.

This function does not work anymore, then the spectra look like the ones above.

Manual:
I now go to the Isoabsorbance Plot and can read out the spectra manually.

Since this also works with the samples that display this atypical spectrum beforehand, there must be a bug in the software somewhere.

I am sure Mrs. Kristen will help..look like software issue..

Is there a way to contact Miss Kirsten directly?

@ martin.adams

Can you help in this regard..

I don't have a way to automatically pull DAD spectra out when looking at the Data Processing section of the CDS 2 Data Analysis software.  However I do have a manual way to pull out multiple spectra from a chromatogram at the same time.  I also know the report templates themselves can automatically pull out the spectra when generating a report.  I'll give examples of both.

Manually pulling spectra from multiple integrated peaks in Data Processing.

1) Activate spectrum extraction

2 & 3) Left click and bracket the peaks to pull spectra from.

4) Click on the check to extra spectra for all included peaks.

Pulling spectra automatically into reports.  In this example I have modified a Short Area report to include spectra from the integrated peaks.  In this case I pulled spectra for my identified peaks.

1 & 2) Drag the appropriate plot into the report template.

Example of a generated report.