Calc

Hi Alex1960

Have a look at the next page in the documentation in the section describing ESTD.

If you include a sample amount in the sequence table and choose ESTD% as the calibration settings section of Specify Report.

Chemstation will calculate the mass percentage by taking the measured amount, divided by the sample amount and multiplied by 100.

/Andy

Hi Andy,

Thank you for the attention to my question!Happy New Year!!!

As you may see, in the formula Agilent has ResponseX as a numerator and as a denumerator. This is meaningless (from math point of view) equation.

Strait calculation would give absolutely incorrect result. What is the point to calculate amount of a sample multiplied by dilution factor and multiplier. In this case we do not need standards at all. Please take a closer look at the equation! When you are working with report, you did not see the formula (real calculations) behind.

Would you please verify (or provide) exact calculation formulas for me?

Thank you

Alex

HI Alex,

I see what you're saying now.  I think the document should have RF=Amount/Response where this is determined from standards.  For each calibration, Chemstation calculates the equation of the line of best fit according to the calibration settings.  This equation is then used to calculate the amount of analyte, which is then corrected using multipliers and dilution factors.  Since each calibration is unique, I can't provide you with an exact equation.  For linear calibrations, the line would have the equation response = a*amount + b, so given a response for a sample, amount can easily be calculated.

Does that make more sense?

/Andy

HI Alex,

I see what you're saying now.  I think the document should have RF=Amount/Response where this is determined from standards.  For each calibration, Chemstation calculates the equation of the line of best fit according to the calibration settings.  This equation is then used to calculate the amount of analyte, which is then corrected using multipliers and dilution factors.  Since each calibration is unique, I can't provide you with an exact equation.  For linear calibrations, the line would have the equation response = a*amount + b, so given a response for a sample, amount can easily be calculated.

Does that make more sense?

/Andy