Hi
After running the APCI tuning checktune, autotun -dual on our HPLC-MS, the MSD cannot find the signal. They more look like noise and fails the tuning.
Can anyone givee a suggestion?
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Hi
After running the APCI tuning checktune, autotun -dual on our HPLC-MS, the MSD cannot find the signal. They more look like noise and fails the tuning.
Can anyone givee a suggestion?
That's more an MS question, so you might want to post your issue in a different forum.
Beside from that, is your MS a Single Quad? G6100 series? Do you get any error message from the software, something like: "Difficulty with spray chamber electronics"? If your spray chamber settings are okay, you have verified that a sufficient amount tuning mix makes it to the nebulizer and you're using a fresh bottle of the correct mix, then I would also make that the corona needle is clean and sharp, the nebulizer is adjusted correctly and the spray shield is clean.
That's more an MS question, so you might want to post your issue in a different forum.
Beside from that, is your MS a Single Quad? G6100 series? Do you get any error message from the software, something like: "Difficulty with spray chamber electronics"? If your spray chamber settings are okay, you have verified that a sufficient amount tuning mix makes it to the nebulizer and you're using a fresh bottle of the correct mix, then I would also make that the corona needle is clean and sharp, the nebulizer is adjusted correctly and the spray shield is clean.
Thanks for replying. I will post this too on MS forum.
Yes. single quad, and sorry forgot the details. The instrument is Agilent LC-MS (1260 HPLC and 6120 MSD). Other details,
- cleaned the source, corona needle with mesh and IPA:water 50:50, every day or before every sequence - checked the nebulizer needle if the tuning mix drops are coming out and also if the spray is coming out - changed the column to see any issue with the column - changed to a different standard material to see any issue with the sample itself - changed the solvent bottles to new solvent mix - refilled the tuning mix bottle to a new one |
I mostly get error message of "Peak width adjust error: the valid range for width gain is -2047 to 2047" and the chromatogram shows bunch of small peaks.
Thanks for sharing the details. Yes, your case would require more of an MS person; hope you will get some advice on the MS forum. Personally I would check the spray chamber parameters and the reported values, but also take a look at the spray shield.