What is the process happening in online UV-spectrophotometer while performing the these activities(Blank correction,Zerocells and medium test) at chemstation software

What is the process happening in online UV-spectrophotometer while performing the these activities(Blank correction,Zerocells and medium test) at chemstation software

  • Hello,

     

    I have moved this post to the Molecular Spectroscopy area where it is more likely that someone with expertise in UV-Vis ChemStation will see it. 

  • Hello naneesh:

     

    During a blank measurement the diode array is electronically set to a zero spectrum and then subtracted from all subsequent intensity spectra to correct for the dark signal.

     

    The Zero Cells command for the multi-cell transport measures a true blank spectrum for cell 1, then measures spectra for the remaining cells. The difference spectra obtained by subtracting the blank spectrum from the individual cell spectra are then subtracted automatically from all subsequent measured spectra to correct for any cell-to-cell optical differences.

     

    The Medium test compares the absorbance of the medium the maximum absorbance set in the dialog box.

     

    Best regards,

     

    Mike

     

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