Anyone experiencing bacterial OD600 samples run on Cary 3500 and only getting half expected result compared to other Agilent UV-Vis models?

Anyone experiencing bacterial OD600 samples run on Cary 3500 and only getting half expected result compared to other Agilent UV-Vis models?

  • Bacterial OD600 measurement is a measurement of scatter - as the amount of bacteria in the sample increases, so does the degree of scatter and so less of the transmitted light reaches the detector (resulting in an apparent increase in absorbance).  This type of measurement is also influenced by how close the detector is to sample - the closer it is the more scattered light is captured and vice-versa.  The detector on the Cary 3500 is very close to the cuvette so this is why the scatter values are lower compared to other Agilent UV-Vis models.

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