I tried to transfer method parameters from Hitachi FL 7000 spectrofluorimeter to agilent cary eclipse in synchronous mode but the two substances that showed two distinct maxima with zero crossing of each at the apex of the other in Hitachi spectrofluorimeter were overlaid with only one peak maxima in cary eclipse.
in Hitachi spectroflourimeter, no clear space in the software to enter delta lambda as cary eclipse spectroflourimeter instead, we enter starting wavelength such as 210 nm and it start switching between monochromators at delta lambda 10 till the set end wavelength
what can be the reason and how to fix it ???