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Wiki Tuning Retention Times in the MassHunter Productivity App
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Table of Contents
  • .X Data file
  • Batch and Sequence menus missing in Drugquant E.02.02. sp2
  • Chemstation E.02.02 SP2 Batch Summary Report does not run.
  • -Collection of MassHunter Productivity App Resources
    • Common Questions about the MassHunter Productivity App
    • Considerations when Adding Custom Methods to the MassHunter Productivity App
    • Create a Custom Report Method for the MassHunter Productivity App
    • Data Review Protocol with Filter Sets in the MassHunter Productivity App
    • Optimizing Efficiency with Compound Groups in the MassHunter Productivity App
    • Result Error Statuses in the MassHunter Productivity App
    • Result Flags in the MassHunter Productivity App
    • Streamlining Sequence Setup in the MassHunter Productivity App
    • Tuning Retention Times in the MassHunter Productivity App
  • Configuring Windows Firewall For GCMSD MassHunter Acquisition
  • DrugQuant is not an option in G1701FA GC/MSD Software until you specify an MS
  • ECM user cannot find data file on ECM server using  GCMS Chemstation.
  • Edit Labname in Report Header, Chemstation E.02.02
  • Error in autotuning tied to missing XML - Masshunter GCMS
  • GCMS MassHunter Installation Guide
  • Host System not Ready - GC/MSD System
  • How to Semi-Quantitate Unknown Compounds in MassHunter Quantitative Analysis
  • Instructions for Enabling Compound Math on B.03.0x-B.07.00
  • Introducing PDF Style Reporting and Copy to LIMS
  • No MS Data Found - G1701FA Chemstation Drug Analysis.
  • QA check report divide by zero error
  • Collection of MSD Productivity ChemStation Software Videos
  • Resetting a Tune file to default 5977 MSD
  • Tune and Vacuum Control Error: Undefined Symbol ADJ_WIDTH or AXIS
  • Wrong quant results shown in QEdit when using TIC as quant signal or importing AMDIS results
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Tuning Retention Times in the MassHunter Productivity App

Created by tannerstevenson tannerstevenson over 2 years ago | Last modified by kristen.sensabaugh kristen.sensabaugh over 1 year ago

For methods with retention times (RTs), the MassHunter Productivity App allows a user to automatically update a method's RT values based on analyzed data. This behavior is especially crucial for the Productivity App because custom methods added to the App become frozen. This means the App will create a private copy of the method so that changes to the original method will not affect the method referenced by the App (for more info, see Considerations when Adding Custom Methods to the MassHunter Productivity App). The ability to automatically maintain valid retention times means that less time is spent managing methods and more time is spent collecting data.

 

The tune function will attempt to update the retention times of all the analyzed compounds with retention time values from the highest concentration calibration standards. Thus, we recommend using this function with calibration samples of sufficient concentration such that all compounds can be found by the integrator. If a new retention time change is greater than the RT window, the app will prompt for confirmation before completing the RT tune. When running an RT tune, the RT window temporarily expands to 200% of the original window to increase the chances of seeing the peak. After applying RT tune run adjustments, the RT window is restored to its original value.

 

The tune functionality is available from either the Setup or Review views:

When started from the Setup view, the App will run the given sequence and method and automatically tune the method with the results. Because the tune functionality depends on calibration samples, the option will only be enabled if the sequence contains calibration samples and compounds are selected.

 

When started from the Review view, the App will use the currently opened results to perform the tune. If there are no Calibration samples in the opened results, the option will not be enabled.

 

Note:

There are some limitations to the tune function. For example, you currently cannot choose to update the RTs of a subset of the analyzed compounds, and you cannot change the updated RT window value. If this functionality will not work with your lab, let us know! We already have several planned upgrades to make this feature more flexible, but the more information we have, the better!

 

Related Links:

Considerations when Adding Custom Methods to the MassHunter Productivity App

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