MassHunter Explorer Normalization

Question

Beyond the Log2 transformation does MH Explorer do any normalization behind the scenes that accounts for different cell counts (like correcting for total TIC abundance)? How is normalization using something like cell count, protein amount or urine creatinine dealt with in Explorer?

howard_sanford · Answer

Hello ChrisL , 
 From what I understand, Explorer currently only has Log2 and LOESS, which are used for batch to batch correction. If more normalizations options are needed, then MassHunter Profiler Professional offers options such as Internal Standard, Percentile Shift, Quantile, Constant Sum, Median Fold Change, Custom R Script and None.