MassHunter Explorer Normalization

Hello ChrisL ,

From what I understand, Explorer currently only has Log2 and LOESS, which are used for batch to batch correction. If more normalizations options are needed, then MassHunter Profiler Professional offers options such as Internal Standard, Percentile Shift, Quantile, Constant Sum, Median Fold Change, Custom R Script and None.

Hello ChrisL ,

From what I understand, Explorer currently only has Log2 and LOESS, which are used for batch to batch correction. If more normalizations options are needed, then MassHunter Profiler Professional offers options such as Internal Standard, Percentile Shift, Quantile, Constant Sum, Median Fold Change, Custom R Script and None.

Thanks. I tried taking the Explorer PFA export into MPP but for some reason it would not recognize data files belonging to the same group as bioreplicates so would not do any statistics. I am going back to the old Profinder to MPP workflow. Explorer is nice but without being able to normalize using such things as protein level it is not much use unless pre-normalization is implemented during the sample prep.