Is it possible in Mashunter to quantitate all chromatogram peaks by one calibration? In the Chemstation Version you can quantitate like this but until now i could not find any solution for this problem in Masshunter.
And another question: If i use an ISTD for quantification, how can i quantify every peak by this?
Thank you
Hello das33 ,
Using an ISTD's response to calibrate other compounds is referred to as Relative ISTD in Quant and Unknowns Analysis. In Quant it is enabled in the method's Global Setup.
In Quant this will only work for defined targets and cannot be used to calculate an estimated amount for any peak, but Unknowns Analysis can be used for this workflow.
Set up your UA file as usual and analyze. After the components are detected, right click on your ISTD and choose the option to Set Hits as ISTDs.
Assign a concentration in the next dialog.
Then in your method on the Target Match tab change the Estimation to Relative ISTD Estimation.
Reanalyze your samples and you will now have an Estimated Conc.
Thank you! I didn´t know that it is referred to Relative ISTD, but now i can run my quantitations. 
In this context i searched also for an solution to calibrate every peak by using an external calibration with different levels. This is also possible in Unknown Analysis? I tried this in Masshunter Quant without being succesfull.
Thanks in advance
Hello das33 ,
With the latest versions of quant the easiest way to use one compound's curve for other compounds is to use the Inherit Calibration feature. Details about this and older options are available in this post.
You can use a single compound's average response factor in Unknowns Analysis, but you must first make a batch with your single compound, multi-level curve. You can then either save just the quant method or add all of the other files of interest and save the batch. After creating your UA file choose the option from the File tab to Import Quantitative Analysis and it will bring in all of the files from the batch along with the target method. Or you can add your samples and then use the Import Target Method option.
For Estimation choose Average RF of closest target.
I should add estimation only works for identified components. If your workflow requires estimates for all components then you can lower your Min match factor to 0 on the Compound Identification tab so that everything is identified, no matter how poorly.
Alternately you could make a personal unknown library by right clicking in the components table and exporting any non-hits to a library.
If you are updating a library you will need to remove it from your method, analyze, and then add it back to the method to force a reload of the library.
