I am trying to explore gain factor for PAH. Unlike many app notes, I have a few extra PAH high boilers with issues in sensitivity on the low end (GC-MS/MS). This is a method transfer from a GC-HRMS so the low calibration was verified with the HRMS instrument. I was reading on how to adjust gain, current at 10 from our training setup, and I cannot see the high boilers in the low standard but in my high standard my most intense peak is 58,000,000. I read a previous forum that had someone quote that 10^8 is ok for MRM, is this true? Any suggestions on what can be done? There was an app note that ran the gain at 20 but others 1 or 10. Please let me know any thoughts.
Thanks in advace,