I have started processing data using Agilent MassHunter Quantitative Software. I have three queries.
1. I have found out there are 4 options to get the calibration curve where i. origin = ignore, ii. origin = include, iii. origin = force and iv. origin = offset. My question is what is/are the criteria to pass the calibration curve?
2. Is there any contribution of the relative response of qualifier(s) of the target and ISTD in the quantitation process?
3. Is there a way to manually enter the relative response of qualifier(s) in the quantitation method if it doesn't get picked up automatically from the data file?