automatically RT

Hi,

how can i change automatically RT in MH 10.1, for example when i change column and etc. I have more than 100 analytes and i dont want to update each analyte manually. Iam using retention time tool for now.

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  • Hello, 

    Are you looking to update the RT in MH Acquistion, Quant, or Qual?

  • You can find the other options to update the RT after loading and editing the method. (Tip: Select a mid- to high-range calibrator before editing the method to use to determine the new RT's.) Once you can see the Method Tasks, there will be an Update menu at the top of the window.

    • Update Retention Times will allow you to choose compounds to update the RT to the current peak's RT at the max height.
    • Update RT from ISTD is helpful if the relative retention times of the target compounds is already in the method. Using this option first sets the ISTD RT to that observed in the selected calibrator and then the target compound is calculated to be ISTD RT x Average RRT.
    • Average Retention Time opens a window, so that you can again choose compounds and specify whether the resulting average RT should be calculated from Cals, QC's and/or weighted Method RT's. 
    • Shift Retention Times again allows you to select the compounds to update by either an absolute time in minutes or a relative shift (a percentage of the RT). For example, if your RT is 2 minutes and you set a relative shift of 90%, the RT would be 1.8 min.

    To complete the list of ways to update the RT in Quant, there is also the Update Retention Time Tool in the Update menu that you mentioned. This is a graphic-based tool that allows the RT to be set by left-clicking on the time you would like to use. This tool will also allow you to move through the compound list in the same window updating RT's based on the peaks found in the window.

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  • You can find the other options to update the RT after loading and editing the method. (Tip: Select a mid- to high-range calibrator before editing the method to use to determine the new RT's.) Once you can see the Method Tasks, there will be an Update menu at the top of the window.

    • Update Retention Times will allow you to choose compounds to update the RT to the current peak's RT at the max height.
    • Update RT from ISTD is helpful if the relative retention times of the target compounds is already in the method. Using this option first sets the ISTD RT to that observed in the selected calibrator and then the target compound is calculated to be ISTD RT x Average RRT.
    • Average Retention Time opens a window, so that you can again choose compounds and specify whether the resulting average RT should be calculated from Cals, QC's and/or weighted Method RT's. 
    • Shift Retention Times again allows you to select the compounds to update by either an absolute time in minutes or a relative shift (a percentage of the RT). For example, if your RT is 2 minutes and you set a relative shift of 90%, the RT would be 1.8 min.

    To complete the list of ways to update the RT in Quant, there is also the Update Retention Time Tool in the Update menu that you mentioned. This is a graphic-based tool that allows the RT to be set by left-clicking on the time you would like to use. This tool will also allow you to move through the compound list in the same window updating RT's based on the peaks found in the window.

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