Hello everyone.
I am new here, and right from the start, I would like to ask for your help. As a newcomer to LC-MS, I have been tasked with developing a method for determining PFAS. After creating the MRM method, it was time to create dynamic MRM since I am analyzing 20 PFAS analytes whose retention times often overlap. However, while automatically generating the dMRM method using the MassHunter software from Agilent, I encountered an error warning "Quant cannot process the selected data file or the Quant result file has been deteled. The missing Quant report: D:/PFAS/Vyvoj/....". I haven´t delete any files from there. The software found all the analytes, and there are no unknown peaks. It just didn’t find three transitions for PFDS, PFHxS, and PFTrDS, specifically from their precursor to the product ion 99, which they all share. Can you please advise me on where the error might be and how I can correctly create the dMRM method using the automatic function?
