6546 Agilent qtof

Hi topa_tof ,

Please share with me a screenshoot of the tuning mix on in positive and then in negative. 

We want to monitor for contamination in positive that could interfere. 

But we also want to evaluate if you are saturating the detector and would need to dilute further.

Regards,

Hello, we did perform a overnight flushing to get rid of any contaminats. For now the spectra looks like : 

positive : 

negative

HI topa_tof ,

Your tune mix is currently contaminated (mass 130m/z) and degraded (118m/z is gone or very low). Also, depending on which QTOF you have, you want to make sure the valve sending nitrogen to the reference nebulizer is in the close position. In negative, you are too concentrated on the mass 601.98m/z, did you dilute the tuning mix?

Please try a new bottle of G1969-85000. First discard the tuning mix that is currently in your bottle B, then clean it 3 times with ACN, then clean it 2-3 times with a small quantity of the new tuning mix.

Please follow these requirements:

Regards,

Hey, 

I am working on 6546 lc qtof with dual ajs esi

I am waiting for new tune mix to come (I just ordered it). But for now the way I have prepared tune mix was as the table below. Even with that i got an error for neg you saw

 The problem with mass 602 is occurring second time and we used different lot for tuning mixs. Also i did rinse a B bottle quite good with heated water then izopropanol then ACN few times (all solvents are highest purity) I think there is more then just faulty tuning mix. 

Also, depending on which QTOF you have, you want to make sure the valve sending nitrogen to the reference nebulizer is in the close position - could you please elaborate more on that?

Do you have any tips form where 130 in pos can come from? 

Thank you for all your suggestions :) 

Hi topa_tof ,

Your recipe is good for the G6546A, but you still need to make sure your are not saturating the detector, the first page on how to prepare the tune mix and what to be carefull with is important. 

For the G6546A, never mind on the valve on the left side of the source, this was for the previous versions of QTOF.

Please start with a new tuning mix solution and make sure you do not have major contamination in positive, if not, then perform your initial tunes in positive and then negative.

Regards,