Hi guys,
Our lab has an Agilent 1290 UPLC attached to a 6475A TQ instrument running on a MassHunter 12.1 Software. In the past 3 days, we failed to get a good tune. It keeps failing we cleaned the everything that we can clean the spray chamber, spray shield, pulled and cleaned the capillary, Flushed the nebulizer, readjusted the nebulizer etc. Our Calibration mix is about 1 month old and has been stored in the refrigerator.
The autotune always fails at MS1 and MS2 lag factors and says it is out of tolerance.
The closest we came was everything passed except the Lag Factor Result table was out of tolerance at the MS1 Scan, Scan speed 0.00 Samples SIM, Step Size 0.10 in positive mode only.
We ordered fresh solution but I really appreciate an input.
I have attached the last Autotune Report for your convenience.
