Hello everyone,
I am facing an issue when trying to perform a System Tune in negative mode of both Quadrupole and TOF following 1 month of the QTOF being off due to a hard drive failure.
Has anyone encountered that error and was able to fix it ?
Backup current tune file before autotune
Running QTOF System Tune in Negative Polarity
Begin Running System Tune
Backup All Parameters
Spraying Calibrant Solution
Disable sureMass optimization
Setup Dual AJS ESI Source to Achieve Stable Signals for Auto Tune
Reset All Parameters to Their Default Values
Coarse Estimate Low Gain Amp Offset Found at 34153
Final Optimum Low Gain Amp Offset Found at 34170
Coarse Estimate Hi Gain Amp Offset Found at 31228
Final Optimum Hi Gain Amp Offset Found at 31174
Waiting for Calibrant to Elute...
Monitoring 601.81 m/z Peak for Intensity and Stability
Signal Intensity and Stability Satisfied Auto Tune Requirements
Performing Coarse TOF Mass Calibration Using 6/10 Calibrant Masses
Successfully Perform Mass Calibration with RMS Deviation (ppm): 0.82
Reset Optics 2 and TOF Parameters to Their Default Values
Signal of the Highest m/z Peaks Below Requirement, Increase MCP to 900
Coarse TOF Resolution - Mirror Mid Optimization
Change Mirror Mid from 1,722.0 to Optimum Value at 1,717.2
Coarse TOF Resolution - Puller Offset Optimization
Change Puller Offset from -32.0 to Optimum Value at -36.9
Performing Coarse TOF Mass Calibration Using 6/10 Calibrant Masses
Successfully Perform Mass Calibration with RMS Deviation (ppm): 0.02
Signal below target do not need to adjust VCap 4000 observed peak height 2296
Adjusting Quad AMU to Produce Single Ion Event at MCP of 720
Attenuate the signal to the highest Quad AMU setting, probably the MCP value is too low
Increasing MCP value by 50 and try again
Signal below target do not need to adjust VCap 4000 observed peak height 18663
Adjusting Quad AMU to Produce Single Ion Event at MCP of 770
Ion arrival frequency 29.9 % too high for the peak height 4483
Increasing MCP value by 25 and try again
Signal below target do not need to adjust VCap 4000 observed peak height 50977
Adjusting Quad AMU to Produce Single Ion Event at MCP of 795
Ion arrival frequency 24.1 % too high for the peak height 4495
Increasing MCP value by 25 and try again
Adjusting VCap to reduce signals to reasonable height 100000
Reduce high mass signals by reduce VCap to 2875 V
Adjusting Quad AMU to Produce Single Ion Event at MCP of 820
Ion arrival frequency 18.1 % too high for the peak height 4072
Increasing MCP value by 25 and try again
Adjusting VCap to reduce signals to reasonable height 100000
Reduce high mass signals by reduce VCap to 2219 V
Adjusting Quad AMU to Produce Single Ion Event at MCP of 845
Coarse optimum Quad AMU Found at 11.6 with Peak Height of 4151
Optimum Quad AMU Found at 1.0 with Arrival Frequency of 4.6 %
Searching MCP to Produce 20 Counts per Single Ion
Counts MCP Frequency Status
04.7 845 7.7 % in Progress
09.7 891 9.6 % in Progress
13.8 922 9.6 % in Progress
24.0 940 8.7 % in Linear Range
Previous Estimation was NOT correct, Readjust Single Ion Event
Adjusting VCap to reduce signals to reasonable height 100000
Reduce high mass signals by reduce VCap to 1750 V
Adjusting Quad AMU to Produce Single Ion Event at MCP of 936
Coarse optimum Quad AMU Found at 11.1 with Peak Height of 4629
Optimum Quad AMU Found at 3.9 with Arrival Frequency of 3.9 %
Searching MCP to Produce 20 Counts per Single Ion
Counts MCP Frequency Status
08.5 936 8.1 % in Progress
09.8 971 7.8 % in Progress
Trying to set MCP to 1001.79694352159, which is out of bounds, set to maximum: 1000
11.6 1000 9.4 % in Progress
Trying to set MCP to 1025.27, which is out of bounds, set to maximum: 1000
13.4 1000 7.9 % in Progress
Trying to set MCP to 1019.86, which is out of bounds, set to maximum: 1000
14.3 1000 8.3 % in Progress
Trying to set MCP to 1017.11, which is out of bounds, set to maximum: 1000
10.1 1000 9.9 % in Progress
Trying to set MCP to 1029.79069767442, which is out of bounds, set to maximum: 1000
12.1 1000 8.0 % in Progress
Trying to set MCP to 1023.82, which is out of bounds, set to maximum: 1000
10.4 1000 8.2 % in Progress
Trying to set MCP to 1028.8438538206, which is out of bounds, set to maximum: 1000
16.1 1000 7.9 % in Linear Range
Trying to set MCP to 1003.93, which is out of bounds, set to maximum: 1000
11.1 1000 9.3 % in Progress
Trying to set MCP to 1026.7, which is out of bounds, set to maximum: 1000
09.6 1000 7.7 % in Progress
Trying to set MCP to 1031.33, which is out of bounds, set to maximum: 1000
15.7 1000 7.7 % in Progress
Trying to set MCP to 1012.82, which is out of bounds, set to maximum: 1000
10.9 1000 8.4 % in Progress
Trying to set MCP to 1027.33, which is out of bounds, set to maximum: 1000
13.7 1000 9.0 % in Progress
Trying to set MCP to 1018.81727574751, which is out of bounds, set to maximum: 1000
11.9 1000 8.8 % in Progress
Trying to set MCP to 1024.40728476821, which is out of bounds, set to maximum: 1000
12.3 1000 8.7 % in Progress
Trying to set MCP to 1023.16, which is out of bounds, set to maximum: 1000
12.4 1000 8.3 % in Progress
Trying to set MCP to 1022.79, which is out of bounds, set to maximum: 1000
16.0 1000 9.6 % in Linear Range
Trying to set MCP to 1003.99, which is out of bounds, set to maximum: 1000
15.7 1000 8.7 % in Progress
Trying to set MCP to 1012.9, which is out of bounds, set to maximum: 1000
10.3 1000 7.5 % in Progress
Restore to previous MCP value 720
Disable sureMass optimization
Stop Spraying Calibrant Solution
System Tune did not complete due to the following reason(s):
Cannot Find the Optimum Detector Gain
Completed QTOF System Tune in Negative Polarity
Many thanks
