Small Peaks and Poor Separation Using 1260 RID

Question

Help with Agilent RID 1260 using a mixed D column, THF as the eluent, analyzing Polystyrene Standards, concentration 2,6 and 10mg/l with a 200ul injection and 1ml/min flow. Peaks are very small and poor separation. Any suggestions

howwhite · Accepted Answer

Tee, 
 
 There is no separation at all. The chromatogram looks as though there is no column installed, which suggests the gel bed has been damaged. This can happen if water or other incompatible solvent was pumped into it. Follow Stephans suggestion below to check out the detector, and if its ok try a new column. 
 
 -Howard

Small Peaks and Poor Separation Using 1260 RID

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