I am new to GPC and currently I am using a Agilent 1260 GPC/SEC Multidetector (LS, RI, and Viscometer) to measure the molecular weight of my standards and samples.
The software I am using now is a GPC software.
I have questions regarding calibration and the Triple analysis.
Firstly, if my standards already had information about the Mw, IV, and I want to do the triple analysis, do I only need to calibrate the system and then to perform the analysis? or do I need to do the universal calibration and column calibration before the analysis?
Secondly, when I perform the triple analysis after the the system calibration, the red boarder in the pictures attached shown and I couldn't proceed the analysis even though I change the curve fit, do you know why it happened and how to fix it?