• State Verified Answer
  • Replies 5 replies
  • Subscribers 94 subscribers
  • Views 946 views
  • Users 0 members are here
Related

Agilent Knowledge Portal

Find maintenance, best practice, and troubleshooting articles.

Agilent Knowledge Portal

Columns for validation of methods

natasa

Hi!

I would like to know if I can use already used columns for validation of methods? I have columns that have already been used for execution of many different methods and I dont know, if they are appropriate for validation of new method. I would like to know if there are any guidelines for useing new, unused or already used columns for validation of methods.

I do have some new columns and I would like to know, if I have to check, if new columns are ok?

  • 0

    Hi

    I think validation depends on performance of column based on your method..

    It needs to check so many parameters for accurate result.

    I attached one ppt, you can go through it..

  • +1

  • 0 in reply to ISH

    Hi.

    While performing validation of method, do you think I need to check three columns with diffrent LOT on the same HPLC sistem with the same mobile faze? Or can I check three diffrent columns as part of repeatabiity between laboratories and repeatability within the laboratory?

    The result is one part of performance of testing robustness. Right?

  • 0 in reply to natasa

    Hi

    Result is part of performance for accuracy of testing of robustness..

  • 0 in reply to ISH

    And results teating amongst laboratories are called Round Robin test to evaluate the performance of accuracy of labs with same method..

Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help