Im trying to run a new methodology to analyz a protein sample, to do  this I need the DAD detector, however, once the sample in injected any signal panel, this problem also occurs with the FLD detector, for this, the autosampler configuration is done, but detector show signal in this chart, but with an “off” sign. Then, how can I solve this problem, how can I get signal from my detector once I injected my sample? 

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