DAD and FLD

Question

Im trying to run a new methodology to analyz a protein sample, to do this I need the DAD detector, however, once the sample in injected any signal panel, this problem also occurs with the FLD detector, for this, the autosampler configuration is done, but detector show signal in this chart, but with an “off” sign. Then, how can I solve this problem, how can I get signal from my detector once I injected my sample?

kbusch · Accepted Answer

Hey Marcela, it seems that the method you have written is missing a wavelength for the DAD signal; therefore, it looks like you are capturing only FLD Signal A. You can right click on the DAD, then click on method. In this section, you can now check the boxes and choose the wavelengths for the DAD that you want to capture. I attached some pictures. It will look similar, but not identical. Then save the method once you have edited it.

DAD and FLD

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