Hi, I am trying to isolate the source of contamination. Is there a way to set up a "no injection" gradient run? I am using a 1290 Infinity II, 6546 QTOF

You should be able to select "no injection".  This may be the most useful for what it looks like you are trying to do.

Thanks! This is useful :)

On the same note of contaminations, is there a way to set up on the system to do an automatic flush/wash without going through the column? 

I’m hoping to use the second set of solvent lines to insert some flushing in between runs with a stronger solvent like MeOH. 

edit: also wondering if it is not possible to set up something automatic, whether or not flushing the column through with 100% MeOH is ok for the column 

Hi SueannL

What SW are you using? You should be able to set up a wash method and include it in your worklist/sequence table. However for it to automatically not pump solvent through the column, you will need a column switching valve set up in your column compartment (and set the valve position in the wash method accordingly). Without a column switching valve, you will need to manually swap the column out with union.

Would you be able to share the model and part # of your column? It is best to check this with our technical experts in the Consumables area.

Hi SueannL

What SW are you using? You should be able to set up a wash method and include it in your worklist/sequence table. However for it to automatically not pump solvent through the column, you will need a column switching valve set up in your column compartment (and set the valve position in the wash method accordingly). Without a column switching valve, you will need to manually swap the column out with union.

Would you be able to share the model and part # of your column? It is best to check this with our technical experts in the Consumables area.

It’s mass hunter software. We don’t have a switching valve so I guess it will have to be manual. Thanks for the advice!