We recently had to have the needle and chamber replaced because of salt clogs. There were salt crystals found around them. Any suggestions for preventing this from happening again? The buffer we are using is 25mM Tris, 1mM EDTA, 1M NaCl, pH8.0.
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We recently had to have the needle and chamber replaced because of salt clogs. There were salt crystals found around them. Any suggestions for preventing this from happening again? The buffer we are using is 25mM Tris, 1mM EDTA, 1M NaCl, pH8.0.
Do you also use acetonitrile somewhere on your solvent cabinet?
No we just use aqueous buffers.
No we just use aqueous buffers.