We recently had to have the needle and chamber replaced because of salt clogs. There were salt crystals found around them. Any suggestions for preventing this from happening again? The buffer we are using is 25mM Tris, 1mM EDTA, 1M NaCl, pH8.0.
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We recently had to have the needle and chamber replaced because of salt clogs. There were salt crystals found around them. Any suggestions for preventing this from happening again? The buffer we are using is 25mM Tris, 1mM EDTA, 1M NaCl, pH8.0.
Sounds like you might have had small leaks during your runs so buffer leaked out and eventually liquid evaporate and only the salts were left. I would make sure i have no leaks and clean the system with water regularly. You might have had a partial clog at some point and it forced liquid out.
Sounds like you might have had small leaks during your runs so buffer leaked out and eventually liquid evaporate and only the salts were left. I would make sure i have no leaks and clean the system with water regularly. You might have had a partial clog at some point and it forced liquid out.
Warming the water helps too.
jhagel is right. You could also put a Dewar vessel with hot water in the solvent cabinet and set the TCC/MCT to a higher temperature.